Journal: Bioactive Materials

Article Title: pH-neutralization strategy to suppress GPCR68 spatiotemporally activates T cells and enhances anti-tumor immunity

doi: 10.1016/j.bioactmat.2026.02.039

Figure Lengend Snippet: GPCR68 as a pH-Sensing regulator in T Cells and generation of GPCR68 fl/fl CD4 Cre mice. (A) Schematic diagram of the effect of pH on T cell GPCR68 as well as tumor. (B) Naïve CD4 + T cells were isolated and activated using anti-CD3 and anti-CD28 using the culture media with varying pH. RT-qPCR was performed to determine the expression of GPCR68 at various pH. (C) Naïve CD4 + T cells were activated with anti-CD3 and anti-CD28 under different pH conditions, and GPCR68 protein expression was assessed by Western blot analysis. (D) To generate conditional knockout (CKO) of GPCR68 in T cells, GPCR68 fl/fl mice were crossed with CD4 Cre mice and generated GPCR68 fl/fl CD4 Cre (CKO). (E) Flow cytometry was used to determine the population of CD4 and CD8 cells in the lymph nodes (LN), thymus (THY), and spleen (SP) at the basal level in CD4 Cre or GPCR68 fl/fl CD4 Cre mice. (F) Flow cytometry was used to determine the population of Foxp3+ Treg cells in the lymph nodes, thymus, and spleen at the basal level in the CD4 Cre or GPCR68 fl/fl CD4 Cre mice. (G-H) The population of F4/80+, CD11c+ (G), and B220+ (H) cells was determined in the lymph nodes and spleen at the basal level in the CD4 Cre or GPCR68 fl/fl CD4 Cre mice. (I-J) Flow cytometry was used to evaluate the CD4 + or CD8 + T cells for the determination of intracellular cytokines IFN-γ+ (I), or TNF-α+ (J) from the spleen and lymph nodes at basal level in the CD4 Cre or GPCR68 fl/fl CD4 Cre mice. Student t-test was performed for comparison between the two groups. Data are mean ± SEM (n = 5), ∗ p < 0.05.

Article Snippet: Naïve T cells were purified from lymph nodes as well as spleens of C57/BL6, CD4 Cre , GPCR68 fl/fl CD4 Cre (CKO) mice by using the mouse naïve CD4 + T Cell Isolation Kit (#130-104-453; Miltenyi Biotec) or naïve CD8 + T Cell Isolation Kit (#130-096-543; Miltenyi Biotec) for negative selection.

Techniques: Isolation, Quantitative RT-PCR, Expressing, Western Blot, Knock-Out, Generated, Flow Cytometry, Comparison

Journal: Bioactive Materials

Article Title: pH-neutralization strategy to suppress GPCR68 spatiotemporally activates T cells and enhances anti-tumor immunity

doi: 10.1016/j.bioactmat.2026.02.039

Figure Lengend Snippet: GPCR68 fl/fl CD4 Cre mice exhibit improved anti-tumor mmune responses. (A-C) Naïve CD4 + T cells were isolated from CD4 Cre or GPCR68 fl/fl CD4 Cre mice and activated using anti-CD3 and anti-CD28 using the culture media under physiologic neutral pH (7.4) or varying pH 6.0, 6.5, or 7.8. Flow cytometry plots showing the expression of IFN-γ and IL-2 in CD4 + T cells from CD4 Cre and GPCR68 fl/fl CD4 Cre mice. Each panel represents the frequency of IFN-γ + and IL-2 + cells. (B) Bar graph summarizing the percentage of IFN-γ + CD4 + T cells at each pH level for CD4 Cre and GPCR68 fl/fl CD4 Cre mice. (C) Bar graph showing the percentage of IL-2 + CD4 + T cells at each pH for CD4 Cre and GPCR68 fl/fl CD4 Cre mice. (D) Experimental timeline depicting tumor induction and treatment protocol in CD4 Cre and GPCR68 fl/fl CD4 Cre mice. (E) Tumor growth curves in CD4 Cre and GPCR68 fl/fl CD4 Cre mice. (F) Tumor weight in CD4 Cre versus GPCR68 fl/fl CD4 Cre mice at the time of harvesting on day 21. (G) Representative images of excised tumors at day 21. (H) Flow cytometric analysis of IFN-γ production by tumor-infiltrating CD4 + and CD8 + T cells. (I) Flow cytometric analysis of TNF-α production by tumor-infiltrating CD4 + and CD8 + T cells. Student t-test was performed for comparison between the two groups. Two-way ANOVA was used for multiple comparisons. Data are mean ± SEM (n = 5). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ns = not significant.

Article Snippet: Naïve T cells were purified from lymph nodes as well as spleens of C57/BL6, CD4 Cre , GPCR68 fl/fl CD4 Cre (CKO) mice by using the mouse naïve CD4 + T Cell Isolation Kit (#130-104-453; Miltenyi Biotec) or naïve CD8 + T Cell Isolation Kit (#130-096-543; Miltenyi Biotec) for negative selection.

Techniques: Isolation, Flow Cytometry, Expressing, Comparison

Journal: Bioactive Materials

Article Title: pH-neutralization strategy to suppress GPCR68 spatiotemporally activates T cells and enhances anti-tumor immunity

doi: 10.1016/j.bioactmat.2026.02.039

Figure Lengend Snippet: Physicochemical properties of BOLT, and BOLT reduces the growth of tumor cells. (A) Schematic of surface double-layer formation and ion release. (B) Negative zeta potential (−1.365 mV) and high conductivity (1.334 mS/cm), confirming colloidal stability and ion release. (C) Uniform particle size (∼1478 nm) across batches. (D) Interfacial pH buffering in PBS. (E) Naïve CD4 + T cells were isolated and activated using anti-CD3 and anti-CD28 using the culture media with 6.0 pH and treated with various doses of BOLT. RT-qPCR was performed to determine the expression of Gpcr68 at various BOLT doses in activated T cells at acidic pH. (F) Anti-CD3 and anti-CD28 activated CD4 + T cells were treated with different doses of BOLT to determine the protein expression of GPCR68 using Western blot. (G-J) CCK8 assay was performed to analyze the effect of various pH on B16, MC38, 143B, and MG63 cell proliferation. (K-L) Effect of various doses of BOLT on the B16 and K7M2 cell growth to determine the IC-50 of BOLT. Error bars represent mean ± SEM. ∗∗ p < 0.01 and ∗ p < 0.05.

Article Snippet: Naïve T cells were purified from lymph nodes as well as spleens of C57/BL6, CD4 Cre , GPCR68 fl/fl CD4 Cre (CKO) mice by using the mouse naïve CD4 + T Cell Isolation Kit (#130-104-453; Miltenyi Biotec) or naïve CD8 + T Cell Isolation Kit (#130-096-543; Miltenyi Biotec) for negative selection.

Techniques: Zeta Potential Analyzer, Isolation, Quantitative RT-PCR, Expressing, Western Blot, CCK-8 Assay

Journal: Bioactive Materials

Article Title: pH-neutralization strategy to suppress GPCR68 spatiotemporally activates T cells and enhances anti-tumor immunity

doi: 10.1016/j.bioactmat.2026.02.039

Figure Lengend Snippet: BOLT activates T cell PI3K-AKT-mTOR pathway to enhance T cell anti-tumor effect. (A) Flow cytometry plots compare IFN-γ and IL-2 expression at pH 7.8 and 6.0 along with various doses of BOLT in CD4 + T cells from CD4 Cre or GPCR68 fl/fl CD4 Cre mice. (B, C) Bar graphs show IFN-γ and IL-2 expression in CD4 + T cells from CD4 Cre or GPCR68 fl/fl CD4 Cre mice. (D) Naïve CD4 + T cells were activated with anti-CD3 and anti-CD28 antibodies and incubated for 3 days with cell culture media of different pH levels. Western blot was performed to determine the phosphorylation of Akt and S6 under acidic conditions (pH 6.5) and alkaline pH (7.8). (E) Activated CD4 + T cells were treated with 0, 0.25, and 0.5 mg/mL doses of BOLT following CD4 + T cells activation at pH 7.8. Western blot analysis showing the phosphorylation of Akt and S6 were observed. (F) CD4 + T cells were activated and treated with BOLT at acidic pH. Western blot analysis was performed to determine the phosphorylation of Akt and S6. Two-way ANOVA was used for multiple comparisons. Experiments were conducted in triplicate. Data are mean ± SEM, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.

Article Snippet: Naïve T cells were purified from lymph nodes as well as spleens of C57/BL6, CD4 Cre , GPCR68 fl/fl CD4 Cre (CKO) mice by using the mouse naïve CD4 + T Cell Isolation Kit (#130-104-453; Miltenyi Biotec) or naïve CD8 + T Cell Isolation Kit (#130-096-543; Miltenyi Biotec) for negative selection.

Techniques: Flow Cytometry, Expressing, Incubation, Cell Culture, Western Blot, Phospho-proteomics, Activation Assay

Journal: STAR Protocols

Article Title: Protocol for potent activation of T cells using BPI-stimulated murine bone marrow-derived cells

doi: 10.1016/j.xpro.2026.104519

Figure Lengend Snippet: Expected results of this protocol Naïve CD4 + T cells cultured for d5 in supernatant of untreated BMDCs (SN NT) or in supernatant of BPI-treated BMDCs (SN BPI). (A) Representative dot blot of flow cytometric analysis of CD62L and CD44 cell surface presentation. (B) IL-22 secretion measured by Luminex technology, n = 4. Data are shown as means ± SEM. Statistical testing was performed using Student`s ratio paired t test.

Article Snippet: Note: If no FACS device is available, the sorting of naïve T cells can be performed using a naïve MACS Sort Kit from Miltenyi Biotec (130-104-453 ).

Techniques: Cell Culture, Dot Blot, Luminex

Journal: iScience

Article Title: BCL6 in T cells promotes type 1 diabetes by redirecting fates of insulin-autoreactive B lymphocytes

doi: 10.1016/j.isci.2026.115990

Figure Lengend Snippet: BCL6 in T cells promotes spontaneous GC formation, anti-insulin B cell infiltration of islets, and spontaneous diabetes development in VH125 SD .NOD mice Cells from spleen, pLNs, mLNs, and pancreas were isolated from 8 to 12-week-old VH125 SD .NOD and VH125 SD .Bcl6 ΔCD4 .NOD mice (genotypes fully defined in ). (A) A representative flow cytometry plot from spleen shows the frequency of insulin-binding B cells (insulin+) identified using biotinylated human insulin/streptavidin fluorochrome as in among total B cells (live singlet CD45 + CD19 + lymphocytes). (B) Diabetes was monitored in cohorts of female VH125 SD .NOD mice ( n = 14, black line) and VH125 SD . Bcl6 ΔCD4 .NOD littermates ( n = 14, purple line) from 10 to 35 weeks of age. Mice were considered diabetic after two consecutive blood glucose readings >250 mg/dL, p < 0.0001, log-rank test. (C–G) Representative flow cytometry plots of pancreatic draining lymph nodes and pancreata gating on anti-insulin B cells as in (A) and as described in for pancreas are shown (C). The frequency of anti-insulin B cells (among total B cells) in (D) spleen, (E) mesenteric lymph nodes (mLNs), (F) pancreatic draining lymph nodes, and (G) pancreata are plotted for individual mice of the indicated genotypes. (H–L) Representative flow cytometry plots from the pancreatic lymph nodes (pLNs) of (H) Tfh cells (live singlet CD45 + CD4 + PD-1 hi CXCR5 hi Foxp3 − lymphocytes) are shown with (I and J) frequencies among total CD4 + Foxp3- CD45+ cells and (K and L) numbers of Tfh cells in pLNs and pancreata plotted for individual mice. (M–Q) Representative flow plots of (M) GC B cells from the pancreatic lymph nodes (live singlet CD45 + CD19 + Fas + GL7 + lymphocytes) with (N and O) frequencies of GC B cells among total B cells and (P and Q) numbers shown for pLNs and pancreata. (C–Q) n = 6–8 mice per group, 5 independent experiments, Mann-Whitney U test, bars representative of mean ± standard deviation. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ns, not significant.

Article Snippet: VH125 SD .NOD Bcl6fl/fl CD4-Cre ± , Backcrossed from transgenic strains gifted by the James W. Thomas and Alexander Dent Laboratories , See also: The Jackson Laboratory, Strains 029288 and 013234.

Techniques: Isolation, Flow Cytometry, Binding Assay, MANN-WHITNEY, Standard Deviation

Journal: iScience

Article Title: BCL6 in T cells promotes type 1 diabetes by redirecting fates of insulin-autoreactive B lymphocytes

doi: 10.1016/j.isci.2026.115990

Figure Lengend Snippet: T cell expression of BCL6 supports enhanced insulitis severity, but is dispensable for tertiary lymphoid structure organization in VH125 SD .NOD mice Pancreata were harvested from female, pre-diabetic VH125 SD .NOD and VH125 SD Bcl6 ΔCD4 .NOD mice at 8–12 and 13–16 weeks of age and were formalin fixed, and paraffin embedded. (A–H) About 10 μm pancreas sections were stained with hematoxylin and eosin (H&E) and blind scored, with individual mice plotted. (A and E) Representative H&E-stained sections are shown, with arrows pointing to islets. All islets were blind scored, with average insulitis scores shown for control VH125 SD .NOD mice (black) and VH125 SD Bcl6 ΔCD4 .NOD mice (purple) at (B) 8–12 weeks and (F) 13–16 weeks. The percentage of islets, which had no lymphocytic infiltrate present were calculated for (C) 8–12 weeks and (G) 13–16 weeks. (D and H) The percentage of islets with each score for all pancreata for 8–12 and 13–16 weeks is shown. (I–L) Pancreas sections from the 8–16-week-old cohort that had the highest insulitis infiltrate ( n = 6 mice per group) were obtained and stained with antibodies reactive against CD3 (T cells) or B220 (B cells). (I) Representative images show “disorganized” T cell-B cell infiltration (top), and “organized” TLS (bottom). Islets were scored separately for T cell (CD3) and B cell (B220) infiltrate as follows: 0 (no T/B infiltrate), 1 (>25% infiltrate), 2 (25%–50% infiltrate), 3 (50%–75% infiltrate), and 4 (>75% infiltrate). Average infiltration score for (J) CD3 + and (K) B220+ cells in islets is shown. (L) Infiltrated islets that scored 2 or above were blind scored as organized (blue) or disorganized (red) in both VH125 SD .NOD mice and VH125 SD Bcl6 ΔCD4 .NOD, n = number of islets scored. (A–L) n = 6–8 mice per group, Bars represent mean ± standard deviation, ns, not significant, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, (B, C, F, G, J, and K) Mann-Whitney U test or (L) chi-square test.

Article Snippet: VH125 SD .NOD Bcl6fl/fl CD4-Cre ± , Backcrossed from transgenic strains gifted by the James W. Thomas and Alexander Dent Laboratories , See also: The Jackson Laboratory, Strains 029288 and 013234.

Techniques: Expressing, Staining, Control, Standard Deviation, MANN-WHITNEY

Journal: iScience

Article Title: BCL6 in T cells promotes type 1 diabetes by redirecting fates of insulin-autoreactive B lymphocytes

doi: 10.1016/j.isci.2026.115990

Figure Lengend Snippet: BCL6 in T cells increases activation and proliferation markers of insulin-binding B cells relative to non-insulin-binding B cells Cells were isolated from 8 to 12-week-old, female, pre-diabetic VH125 SD .NOD with and without Cd4 -Cre Bcl6 deletion from (A–G) spleen, pancreatic lymph nodes (pLNs) and pancreata. (A–C) Representative flow cytometry plots show Ki67 staining overlays of insulin-binding (left) or non-insulin-binding (right) B cells (identified as in ) from each genotype within live singlet GL7- CD95- CD45 + CD19 + lymphocytes in spleen (A), pancreatic lymph nodes (B), and pancreata (C). (D) Non-GC (Fas − GL7 − ) B cells were further gated on insulin-binding (ins+) and non-insulin-binding (ins−) and the frequency of cells that were Ki67+ (a marker of proliferation). (E–G) Insulin+ or insulin− B cell expression of (E) CD86 (T cell co-stimulatory molecule), (F) CD44 (activation marker), and (G) CD69 (activation marker) is shown for spleen (left), pancreatic lymph nodes (middle), and pancreata (right), with n = 6–8 individual mice plotted per group. (D–G) Bars represent mean ± standard deviation. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ns = not significant, Kruskal-Wallis test with post hoc test of multiple comparisons. All other comparisons not shown are not significant.

Article Snippet: VH125 SD .NOD Bcl6fl/fl CD4-Cre ± , Backcrossed from transgenic strains gifted by the James W. Thomas and Alexander Dent Laboratories , See also: The Jackson Laboratory, Strains 029288 and 013234.

Techniques: Activation Assay, Binding Assay, Isolation, Flow Cytometry, Staining, Marker, Expressing, Standard Deviation

Journal: iScience

Article Title: BCL6 in T cells promotes type 1 diabetes by redirecting fates of insulin-autoreactive B lymphocytes

doi: 10.1016/j.isci.2026.115990

Figure Lengend Snippet: BCL6 loss in CD4 + T cells impairs CD4 + T cell activation and promotes Treg formation in some sites but does not affect anti-insulin B cell proliferation in the pancreas CD4 + T cells were isolated from 12 to 14 week-old female donors and ∼5 × 10 6 BCL6+ or ΔBCL6 CD4 + T cells were CellTrace Violet (CTV)-labeled and adoptively transferred into 8–12 week-old VH125 SD .Bcl6 ΔCD4 .NOD recipients. Seven days after transfer, spleen, pancreatic lymph nodes, and pancreata were assayed as follows. (A) Experimental schematic. (B) (Left) Representative flow plots of CD4 + CTV+ labeled cells in FMO control, spleen, pancreatic lymph nodes, and pancreas. (Right) The proportions of CTV+ labeled cells in spleen, pancreatic lymph nodes, and pancreata are shown for individual recipients following BCL6+ or ΔBCL6 CD4 + transfers. (C–F) CTV+ (transferred) or CTV- (endogenous, non-transferred) CD4 + T cells were assessed regarding: (C) CD44 MFI levels, (D) % CD69 + , (E) % Tfh (CXCR5+ PD-1+), and (F) % Treg (FoxP3+). (G–H) % GC B cells (CD95 + GL7+) is shown among total B cells (CD19 + B220+ CD45 + live cells) and (H) % Ki67+ among non-GC (CD95 − GL7 − CD19 + B220+ CD45 + live) B cells in either insulin-binding (INS+) or non-insulin binding (INS−) B cells. (B–H) n = 5–6 mice per group, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ns, not significant, Kruskal-Wallis test with post hoc test of multiple comparisons (B–F and H) or Mann-Whitney U test (G) was used for analyses. All other comparisons not shown are not significant. Bars represent mean ± standard deviation.

Article Snippet: VH125 SD .NOD Bcl6fl/fl CD4-Cre ± , Backcrossed from transgenic strains gifted by the James W. Thomas and Alexander Dent Laboratories , See also: The Jackson Laboratory, Strains 029288 and 013234.

Techniques: Activation Assay, Isolation, Labeling, Control, Binding Assay, MANN-WHITNEY, Standard Deviation

Journal: iScience

Article Title: BCL6 in T cells promotes type 1 diabetes by redirecting fates of insulin-autoreactive B lymphocytes

doi: 10.1016/j.isci.2026.115990

Figure Lengend Snippet: Insulin-binding B cells exist in extrafollicular niches and skew toward CD11c + T-bet + and CD11b + CD11c + atypical B cell subsets, some of which are reduced by loss of Bcl6 Spleen, pancreatic draining lymph nodes, and pancreata were harvested from pre-diabetic, 8–12-week-old VH125 SD .NOD mice. (A) Around 10 μm sections of spleen form VH125 SD .NOD underwent immunofluorescence staining with IgD, Ki67, insulin, and CD3. Representative merge image shown on left with identified borders of B cell zone (BCZ), T cell zone (TCZ), germinal center (GC) and extrafollicular area (EF). Circled cells are representative insulin-autoreactive B cells, which express both IgD and insulin. Quantification of % insulin+ B cells in predefined niches on the left graph. Quantification of insulin+ IgD + colocalization, or insulin+ IgD-. n = 4–6 individual splenic sections per group. (B) Representative flow plots show CD11c+/CD11b+ cells among B220+ CD19 + live singlet lymphocytes for both non-insulin-reactive (insulin−, left) and insulin-autoreactive (insulin+, middle, right) B cell populations in the pancreas. (C) Quantification of the proportion of CD11c+ CD11b+ B cells in pancreata for both insulin− and insulin+ B cells for VH125 SD (black) and VH125 SD Bcl6 ΔCD4 (purple) mice. (D) Representative flow plots of CD11c+/Tbet+ cells among B220+ CD19 + live singlet lymphocytes for both insulin− and insulin+ B cell populations in the pancreas. (E) Quantification of the proportion of CD11c+/Tbet+ cells among both insulin− and insulin+ B cells in the pancreas of VH125 SD (black) and VH125 SD Bcl6 ΔCD4 (purple) mice. (F and G) Quantification of the proportion of CD11c+/Tbet+ B cells and CD11b+/CD11c+ B cells in pancreatic draining lymph nodes (F) and spleen (G). (A–G) One-way ANOVA (A, left), Mann-Whitney U test (A, right) (A) or Kruskal-Wallis test (C–G) with post hoc multiple comparison test were used, n = 4–8 mice per group. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, ns, not significant. All other comparisons not shown are not significant. Bars represent mean ± standard deviation.

Article Snippet: VH125 SD .NOD Bcl6fl/fl CD4-Cre ± , Backcrossed from transgenic strains gifted by the James W. Thomas and Alexander Dent Laboratories , See also: The Jackson Laboratory, Strains 029288 and 013234.

Techniques: Binding Assay, Immunofluorescence, Staining, MANN-WHITNEY, Comparison, Standard Deviation

Journal: iScience

Article Title: BCL6 in T cells promotes type 1 diabetes by redirecting fates of insulin-autoreactive B lymphocytes

doi: 10.1016/j.isci.2026.115990

Figure Lengend Snippet: BCL6 loss in T cells promotes formation of a phenotypically defined atypical memory B cell population in the pancreas of VH125 SD .NOD mice Pancreata were harvested from pre-diabetic, 8–12-week-old Bcl6 -sufficient (VH125 SD ) and Bcl6 -deficient (VH125 SD Bcl6 ΔCD4 .NOD) mice ( n = 12–14 mice) and flow cytometry staining was performed using the panel of markers in and (D). B220+ CD19 + CD45 + live singlets were concatenated and normalized via CyCombine and concatenated, with genotype metadata encoded. (A) t-SNE was used to perform dimensionality reduction based on phenotypic marker expression profiles. Clusters were manually defined as indicated. (B) The cluster frequency is shown for each genotype, with individual mice plotted and means indicated. ∗ p < 0.05, Kruskal-Wallis test with post hoc multiple comparison test. Bars represent mean ± standard deviation. (C) A rainbow intensity scale indicates expression levels of the phenotypic markers indicated at the top of each t-SNE plot. Insulin-binding, CD45, B220, CD19, and viability dye markers were omitted from the t-SNE analysis given they were used in parent population gating upstream of t-SNE analysis. (D) Heatmap shows relative expression of the indicated markers for each cluster defined as in (A) using a log-like arcsinh scale and appropriate arcsinh factors.

Article Snippet: VH125 SD .NOD Bcl6fl/fl CD4-Cre ± , Backcrossed from transgenic strains gifted by the James W. Thomas and Alexander Dent Laboratories , See also: The Jackson Laboratory, Strains 029288 and 013234.

Techniques: Flow Cytometry, Staining, Marker, Expressing, Comparison, Standard Deviation, Binding Assay

Journal: STAR Protocols

Article Title: Protocol for the identification of recent HIV infection in newly diagnosed individuals

doi: 10.1016/j.xpro.2026.104504

Figure Lengend Snippet: Sequential decision-tree for identifying recent HIV infections using a multi-assay algorithm (MAA) Hierarchical integration of HIV infection biomarkers: preserved immune function (CD4 + >200 cells/μL), active viral replication (pVL >1,000 copies/mL), immature antibody avidity (AI <0.7), and limited viral genetic diversity (ambiguity index <0.47%) classifies recent infections (≤12 months post-seroconversion).

Article Snippet: Portable POC imaging cytometers, e . g ., PimaTM CD4 analyzer (Abbott), offer accurate measurements comparable to standard flow cytometry , for reliable CD4 monitoring in decentralized settings.

Techniques: Infection

Journal: STAR Protocols

Article Title: Protocol for potent activation of T cells using BPI-stimulated murine bone marrow-derived cells

doi: 10.1016/j.xpro.2026.104519

Figure Lengend Snippet: Flow cytometric quality control of CD4 + T-cell sort via MACS technique

Article Snippet: Naive CD4 + T-Cell Isolation Kit, mouse , Miltenyi Biotec , Cat# 130-104-453.

Techniques: Control

Journal: STAR Protocols

Article Title: Protocol for potent activation of T cells using BPI-stimulated murine bone marrow-derived cells

doi: 10.1016/j.xpro.2026.104519

Figure Lengend Snippet: Gating strategy for further isolation of CD4 + T-cell populations via fluorescence-activated cell sorting

Article Snippet: Naive CD4 + T-Cell Isolation Kit, mouse , Miltenyi Biotec , Cat# 130-104-453.

Techniques: Isolation, Fluorescence, FACS

Journal: STAR Protocols

Article Title: Protocol for potent activation of T cells using BPI-stimulated murine bone marrow-derived cells

doi: 10.1016/j.xpro.2026.104519

Figure Lengend Snippet: Expected results of this protocol Naïve CD4 + T cells cultured for d5 in supernatant of untreated BMDCs (SN NT) or in supernatant of BPI-treated BMDCs (SN BPI). (A) Representative dot blot of flow cytometric analysis of CD62L and CD44 cell surface presentation. (B) IL-22 secretion measured by Luminex technology, n = 4. Data are shown as means ± SEM. Statistical testing was performed using Student`s ratio paired t test.

Article Snippet: Naive CD4 + T-Cell Isolation Kit, mouse , Miltenyi Biotec , Cat# 130-104-453.

Techniques: Cell Culture, Dot Blot, Luminex